4IXJ
The structure of PilJ, a Type IV pilin from Clostridium difficile
Summary for 4IXJ
| Entry DOI | 10.2210/pdb4ixj/pdb |
| Related | 1OQV 3SOT |
| Descriptor | Fimbrial protein (Pilin), ZINC ION, GLYCEROL, ... (4 entities in total) |
| Functional Keywords | filament formation, cell adhesion |
| Biological source | Clostridium difficile |
| Total number of polymer chains | 2 |
| Total formula weight | 64236.17 |
| Authors | Piepenbrink, K.H.,Sundberg, E.J.,Snyder, G.A. (deposition date: 2013-01-25, release date: 2014-01-01, Last modification date: 2024-02-28) |
| Primary citation | Piepenbrink, K.H.,Maldarelli, G.A.,de la Pena, C.F.,Mulvey, G.L.,Snyder, G.A.,De Masi, L.,von Rosenvinge, E.C.,Gunther, S.,Armstrong, G.D.,Donnenberg, M.S.,Sundberg, E.J. Structure of Clostridium difficile PilJ Exhibits Unprecedented Divergence from Known Type IV Pilins. J.Biol.Chem., 289:4334-4345, 2014 Cited by PubMed Abstract: Type IV pili are produced by many pathogenic Gram-negative bacteria and are important for processes as diverse as twitching motility, cellular adhesion, and colonization. Recently, there has been an increased appreciation of the ability of Gram-positive species, including Clostridium difficile, to produce Type IV pili. Here we report the first three-dimensional structure of a Gram-positive Type IV pilin, PilJ, demonstrate its incorporation into Type IV pili, and offer insights into how the Type IV pili of C. difficile may assemble and function. PilJ has several unique structural features, including a dual-pilin fold and the incorporation of a structural zinc ion. We show that PilJ is incorporated into Type IV pili in C. difficile and present a model in which the incorporation of PilJ into pili exposes the C-terminal domain of PilJ to create a novel interaction surface. PubMed: 24362261DOI: 10.1074/jbc.M113.534404 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (1.983 Å) |
Structure validation
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