4IXJ

The structure of PilJ, a Type IV pilin from Clostridium difficile

4IXJ の概要

• エントリーDOI: 10.2210/pdb4ixj/pdb
• 関連するPDBエントリー: 1OQV 3SOT
• 分子名称: Fimbrial protein (Pilin), ZINC ION, GLYCEROL, ... (4 entities in total)
• 機能のキーワード: filament formation, cell adhesion
• 由来する生物種: Clostridium difficile
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 64236.17

構造登録者

Piepenbrink, K.H.,Sundberg, E.J.,Snyder, G.A. (登録日: 2013-01-25, 公開日: 2014-01-01, 最終更新日: 2024-02-28)

主引用文献

Piepenbrink, K.H.,Maldarelli, G.A.,de la Pena, C.F.,Mulvey, G.L.,Snyder, G.A.,De Masi, L.,von Rosenvinge, E.C.,Gunther, S.,Armstrong, G.D.,Donnenberg, M.S.,Sundberg, E.J.
Structure of Clostridium difficile PilJ Exhibits Unprecedented Divergence from Known Type IV Pilins.
J.Biol.Chem., 289:4334-4345, 2014

PubMed Abstract: Type IV pili are produced by many pathogenic Gram-negative bacteria and are important for processes as diverse as twitching motility, cellular adhesion, and colonization. Recently, there has been an increased appreciation of the ability of Gram-positive species, including Clostridium difficile, to produce Type IV pili. Here we report the first three-dimensional structure of a Gram-positive Type IV pilin, PilJ, demonstrate its incorporation into Type IV pili, and offer insights into how the Type IV pili of C. difficile may assemble and function. PilJ has several unique structural features, including a dual-pilin fold and the incorporation of a structural zinc ion. We show that PilJ is incorporated into Type IV pili in C. difficile and present a model in which the incorporation of PilJ into pili exposes the C-terminal domain of PilJ to create a novel interaction surface.

PubMed: 24362261
DOI: 10.1074/jbc.M113.534404

実験手法

X-RAY DIFFRACTION (1.983 Å)