4IWY

SeMet-substituted RimK structure

4IWY の概要

• エントリーDOI: 10.2210/pdb4iwy/pdb
• 関連するPDBエントリー: 4IWX
• 分子名称: Ribosomal protein S6 modification protein, ADENOSINE-5'-DIPHOSPHATE, GLUTAMIC ACID, ... (5 entities in total)
• 機能のキーワード: atp-grasp fold, ligase
• 由来する生物種: Escherichia coli
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 36223.63

構造登録者

Shi, D.,Zhao, G.,Jin, Z.,Allewell, N.M.,Tuchman, M. (登録日: 2013-01-24, 公開日: 2013-05-08, 最終更新日: 2024-11-06)

主引用文献

Zhao, G.,Jin, Z.,Wang, Y.,Allewell, N.M.,Tuchman, M.,Shi, D.
Structure and function of Escherichia coli RimK, an ATP-grasp fold, l-glutamyl ligase enzyme.
Proteins, 81:1847-1854, 2013

PubMed Abstract: We report herein the crystal structure of Escherichia coli RimK at a resolution of 2.85 Å, an enzyme that catalyzes the post-translational addition of up to 15 C-terminal glutamate residues to ribosomal protein S6. The structure belongs to the ATP-grasp superfamily and is organized as a tetramer, consistent with gel filtration analysis. Each subunit consists of three distinct structural domains and the active site is located in the cleft between these domains. The catalytic reaction appears to occur at the junction between the three domains as ATP binds between the B and C domains, and other substrates bind nearby.

PubMed: 23609986
DOI: 10.1002/prot.24311

実験手法

X-RAY DIFFRACTION (2.9 Å)