4IWB

Novel Fold of FliC/FliS Fusion Protein

4IWB の概要

• エントリーDOI: 10.2210/pdb4iwb/pdb
• 関連するPDBエントリー: 1ORY
• 分子名称: FliC, FliS chimera (2 entities in total)
• 機能のキーワード: novel fold, motor protein
• 由来する生物種: Aquifex aeolicus; 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 38584.56

構造登録者

Faham, S. (登録日: 2013-01-23, 公開日: 2013-12-11, 最終更新日: 2024-02-28)

主引用文献

Skorupka, K.,Han, S.K.,Nam, H.J.,Kim, S.,Faham, S.
Protein design by fusion: implications for protein structure prediction and evolution.
Acta Crystallogr.,Sect.D, 69:2451-2460, 2013

PubMed Abstract: Domain fusion is a useful tool in protein design. Here, the structure of a fusion of the heterodimeric flagella-assembly proteins FliS and FliC is reported. Although the ability of the fusion protein to maintain the structure of the heterodimer may be apparent, threading-based structural predictions do not properly fuse the heterodimer. Additional examples of naturally occurring heterodimers that are homologous to full-length proteins were identified. These examples highlight that the designed protein was engineered by the same tools as used in the natural evolution of proteins and that heterodimeric structures contain a wealth of information, currently unused, that can improve structural predictions.

PubMed: 24311586
DOI: 10.1107/S0907444913022701

実験手法

X-RAY DIFFRACTION (1.75 Å)