Crystal structure of an O2-tolerant [NiFe]-hydrogenase from Ralstonia eutropha in its as-isolated form - oxidized state 1

Summary for 4IUB

Related4IUC 4IUD
DescriptorUptake hydrogenase large subunit, Uptake hydrogenase small subunit, NI-FE OXIDIZED ACTIVE CENTER, ... (10 entities in total)
Functional Keywords[nife] hydrogenase, knallgas bacteria, proteobacteria, aerobic hydrogen bacteria, hydrogen catalysis, metalloenzyme, metalloprotein catalytic center, nickel-iron cofactor, bimetallic, ni-fe active site, iron-sulfur cluster, [4fe-3s] cluster, [3fe-4s] cluster, [4fe-4s] cluster, reduced state, oxidized state, oxygen-tolerant hydrogenase, membrane-bound, oxidoreductase
Biological sourceRalstonia eutropha
Cellular locationCell membrane; Peripheral membrane protein P31891 P31892
Total number of polymer chains2
Total molecular weight106454.89
Frielingsdorf, S.,Schmidt, A.,Fritsch, J.,Lenz, O.,Scheerer, P. (deposition date: 2013-01-20, release date: 2014-04-02, Last modification date: 2017-11-15)
Primary citation
Frielingsdorf, S.,Fritsch, J.,Schmidt, A.,Hammer, M.,Lowenstein, J.,Siebert, E.,Pelmenschikov, V.,Jaenicke, T.,Kalms, J.,Rippers, Y.,Lendzian, F.,Zebger, I.,Teutloff, C.,Kaupp, M.,Bittl, R.,Hildebrandt, P.,Friedrich, B.,Lenz, O.,Scheerer, P.
Reversible [4Fe-3S] cluster morphing in an O2-tolerant [NiFe] hydrogenase.
Nat.Chem.Biol., 10:378-385, 2014
PubMed: 24705592 (PDB entries with the same primary citation)
DOI: 10.1038/nchembio.1500
MImport into Mendeley
Experimental method

Structure validation

RfreeClashscoreRamachandran outliersSidechain outliersRSRZ outliers0.174400.1%2.7%MetricValuePercentile RanksWorseBetterPercentile relative to all X-ray structuresPercentile relative to X-ray structures of similar resolution

More Asymmetric unit images

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Molmil generated image of 4iub
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