4ITV

Structure of a 16 nm protein cage designed by fusing symmetric oligomeric domains, triple mutant, P212121 form

4ITV の概要

• エントリーDOI: 10.2210/pdb4itv/pdb
• 関連するPDBエントリー: 3VDX 4D9J 4IQ4 4IVJ
• 分子名称: Non-haem bromoperoxidase BPO-A2, Matrix protein 1 (1 entity in total)
• 機能のキーワード: protein design, bionanotechnology, protein assembly, symmetric oligomeric domains, biomaterials, oxidoreductase
• 由来する生物種: Streptomyces aureofaciens; 詳細
• 細胞内の位置: Virion membrane ; Peripheral membrane protein ; Cytoplasmic side : P03485
• タンパク質・核酸の鎖数: 12
• 化学式量合計: 603376.31

構造登録者

Lai, Y.-T.,Sawaya, M.R.,Yeates, T.O. (登録日: 2013-01-18, 公開日: 2013-07-24, 最終更新日: 2023-09-20)

主引用文献

Lai, Y.T.,Tsai, K.L.,Sawaya, M.R.,Asturias, F.J.,Yeates, T.O.
Structure and flexibility of nanoscale protein cages designed by symmetric self-assembly.
J.Am.Chem.Soc., 135:7738-7743, 2013

PubMed Abstract: Designing protein molecules that self-assemble into complex architectures is an outstanding goal in the area of nanobiotechnology. One design strategy for doing this involves genetically fusing together two natural proteins, each of which is known to form a simple oligomer on its own (e.g., a dimer or trimer). If two such components can be fused in a geometrically predefined configuration, that designed subunit can, in principle, assemble into highly symmetric architectures. Initial experiments showed that a 12-subunit tetrahedral cage, 16 nm in diameter, could be constructed following such a procedure [Padilla, J. E.; et al. Proc. Natl. Acad. Sci. U.S.A. 2001, 98, 2217; Lai, Y. T.; et al. Science 2012, 336, 1129]. Here we characterize multiple crystal structures of protein cages constructed in this way, including cages assembled from two mutant forms of the same basic protein subunit. The flexibilities of the designed assemblies and their deviations from the target model are described, along with implications for further design developments.

PubMed: 23621606
DOI: 10.1021/ja402277f

実験手法

X-RAY DIFFRACTION (3.598 Å)