4IT7
Crystal structure of Al-CPI
4IT7 の概要
エントリーDOI | 10.2210/pdb4it7/pdb |
分子名称 | CPI (2 entities in total) |
機能のキーワード | cpi, cystatin, hydrolase inhibitor |
由来する生物種 | Ascaris lumbricoides (common roundworm) |
タンパク質・核酸の鎖数 | 4 |
化学式量合計 | 48182.71 |
構造登録者 | |
主引用文献 | Mei, G.,Dong, J.,Li, Z.,Liu, S.,Liu, Y.,Sun, M.,Liu, G.,Su, Z.,Liu, J. Structural Basis for the Immunomodulatory Function of Cysteine Protease Inhibitor from Human Roundworm Ascaris lumbricoides. Plos One, 9:e96069-e96069, 2014 Cited by PubMed Abstract: Immunosuppression associated with infections of nematode parasites has been documented. Cysteine protease inhibitor (CPI) released by the nematode parasites is identified as one of the major modulators of host immune response. In this report, we demonstrated that the recombinant CPI protein of Ascaris lumbricoides (Al-CPI) strongly inhibited the activities of cathepsin L, C, S, and showed weaker effect to cathepsin B. Crystal structure of Al-CPI was determined to 2.1 Å resolution. Two segments of Al-CPI, loop 1 and loop 2, were proposed as the key structure motifs responsible for Al-CPI binding with proteases and its inhibitory activity. Mutations at loop 1 and loop 2 abrogated the protease inhibition activity to various extents. These results provide the molecular insight into the interaction between the nematode parasite and its host and will facilitate the development of anthelmintic agents or design of anti-autoimmune disease drugs. PubMed: 24781326DOI: 10.1371/journal.pone.0096069 主引用文献が同じPDBエントリー |
実験手法 | X-RAY DIFFRACTION (2.1 Å) |
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