4IR7

Crystal Structure of Mtb FadD10 in Complex with Dodecanoyl-AMP

4IR7 の概要

• エントリーDOI: 10.2210/pdb4ir7/pdb
• 分子名称: Long chain fatty acid CoA ligase FadD10, 5'-O-[(S)-(dodecanoyloxy)(hydroxy)phosphoryl]adenosine, MAGNESIUM ION, ... (4 entities in total)
• 機能のキーワード: open conformation, structural genomics, tb structural genomics consortium, tbsgc, transferase
• 由来する生物種: Mycobacterium tuberculosis
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 57281.32

構造登録者

Liu, Z.,Wang, F.,Sacchettini, J.C.,TB Structural Genomics Consortium (TBSGC) (登録日: 2013-01-14, 公開日: 2013-05-08, 最終更新日: 2024-02-28)

主引用文献

Liu, Z.,Ioerger, T.R.,Wang, F.,Sacchettini, J.C.
Structures of Mycobacterium tuberculosis FadD10 protein reveal a new type of adenylate-forming enzyme.
J.Biol.Chem., 288:18473-18483, 2013

PubMed Abstract: Mycobacterium tuberculosis has a group of 34 FadD proteins that belong to the adenylate-forming superfamily. They are classified as either fatty acyl-AMP ligases (FAALs) or fatty acyl-CoA ligases based on sequence analysis. FadD10, involved in the synthesis of a virulence-related lipopeptide, was mis-annotated as a fatty acyl-CoA ligase; however, it is in fact a FAAL that transfers fatty acids to an acyl carrier protein (Rv0100). In this study, we have determined the structures of FadD10 in both the apo-form and the complexed form with dodecanoyl-AMP, where we see for the first time an adenylate-forming enzyme that does not adopt a closed conformation for catalysis. Indeed, this novel conformation of FadD10, facilitated by its unique inter-domain and intermolecular interactions, is critical for the enzyme to carry out the acyl transfer onto Rv0100 rather than coenzyme A. This contradicts the existing model of FAALs that rely on an insertion motif for the acyltransferase specificity and thus makes FadD10 a new type of FAAL. We have also characterized the fatty acid preference of FadD10 through biological and structural analyses, and the data indicate long chain saturated fatty acids as the biological substrates of the enzyme.

PubMed: 23625916
DOI: 10.1074/jbc.M113.466912

実験手法

X-RAY DIFFRACTION (2.8 Å)