4IOY

Structure of the Spt16 Middle Domain Reveals Functional Features of the Histone Chaperone FACT

Summary for 4IOY

• Entry DOI: 10.2210/pdb4ioy/pdb
• Related: 2GCL 3FSS 3GYP 3TO1
• Descriptor: FACT complex subunit SPT16, PHOSPHATE ION (3 entities in total)
• Functional Keywords: spt16, fact, double pleckstrin homology domain, h3-h4 histones, transcription
• Biological source: Saccharomyces cerevisiae (Baker's yeast)
• Cellular location: Nucleus: P32558
• Total number of polymer chains: 1
• Total formula weight: 33161.69

Authors

Kemble, D.J.,Hill, C.P. (deposition date: 2013-01-08, release date: 2013-02-20, Last modification date: 2024-02-28)

Primary citation

Kemble, D.J.,Whitby, F.G.,Robinson, H.,McCullough, L.L.,Formosa, T.,Hill, C.P.
Structure of the Spt16 Middle Domain Reveals Functional Features of the Histone Chaperone FACT.
J.Biol.Chem., 288:10188-10194, 2013

PubMed Abstract: The histone chaperone FACT is an essential and abundant heterodimer found in all eukaryotes. Here we report a crystal structure of the middle domain of the large subunit of FACT (Spt16-M) to reveal a double pleckstrin homology architecture. This structure was found previously in the Pob3-M domain of the small subunit of FACT and in the related histone chaperone Rtt106, although Spt16-M is distinguished from these structures by the presence of an extended α-helix and a C-terminal addition. Consistent with our finding that the double pleckstrin homology structure is common to these three histone chaperones and reports that Pob3 and Rtt106 double pleckstrin homology domains bind histones H3-H4, we also find that Spt16-M binds H3-H4 with low micromolar affinity. Our structure provides a framework for interpreting a large body of genetic data regarding the physiological functions of FACT, including the identification of potential interaction surfaces for binding histones or other proteins.

PubMed: 23417676
DOI: 10.1074/jbc.C113.451369

Experimental method

X-RAY DIFFRACTION (1.945 Å)