4IMB

Structure of strictosidine synthase in complex with 2-(1-methyl-1H-indol-3-yl)ethanamine

4IMB の概要

• エントリーDOI: 10.2210/pdb4imb/pdb
• 分子名称: Strictosidine synthase, 2-(1-methyl-1H-indol-3-yl)ethanamine (3 entities in total)
• 機能のキーワード: strictosidine synthase, strictosidine, lyase-lyase inhibitor complex, lyase/lyase inhibitor
• 由来する生物種: Rauvolfia serpentina (devilpepper)
• 細胞内の位置: Vacuole: P68175
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 67561.45

構造登録者

Stoeckigt, J.,Fangrui, W.,Wang, M.,Rajendran, C. (登録日: 2013-01-02, 公開日: 2014-01-29, 最終更新日: 2024-10-16)

主引用文献

Zhu, H.,Kercmar, P.,Wu, F.,Rajendran, C.,Sun, L.,Wang, M.,Stockigt, J.
Using Strictosidine Synthase to Prepare Novel Alkaloids.
Curr.Med.Chem., 2015

PubMed Abstract: The Pictet-Spenglerasestrictosidine synthase (STR) has been characterized as the central enzyme in the biosynthesis of around 2000 monoterpenoid indole alkaloids in plants. In the light of a high therapeutic value and huge scaffold diversity these alkaloids represent, STR as an enzyme has attracted great attentions in recent years, intending to be utilized in the formation of new interesting alkaloids with unusual substitution pattern or even with novel scaffolds. For outlining the application potential that STR possesses, together with insight into the reaction mechanism catalyzed by STR, strategies and methods for exploring the applicability of STR have been updated in this article by taking R. serpertina STR(RS-STR) and C. roseus.STR (CR-STR) as representative models, followed by introducing the latest released complex structures of RS-STR with new substrates. Examples provided here, including substrate scaffold tailoring, X-ray crystal complex structure comparison, protein engineering and biosynthetic pathway reprogramming, pave the way to finally construct novel alkaloids libraries by chemo-enzymatic approaches.

PubMed: 25850764

実験手法

X-RAY DIFFRACTION (2.703 Å)