4ILU

Crystal structure of Mycobacterium tuberculosis CarD

4ILU の概要

• エントリーDOI: 10.2210/pdb4ilu/pdb
• 関連するPDBエントリー: 4MFR
• 分子名称: RNA polymerase-binding transcription factor CarD, SULFATE ION (3 entities in total)
• 機能のキーワード: tudor like domain, five helical fold, rna polymerase binding, transcription regulation, transcription
• 由来する生物種: Mycobacterium tuberculosis
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 19660.96

構造登録者

Thakur, K.G.,Kaur, G. (登録日: 2013-01-01, 公開日: 2013-10-30, 最終更新日: 2024-05-29)

主引用文献

Kaur, G.,Dutta, D.,Thakur, K.G.
Crystal structure of Mycobacterium tuberculosis CarD, an essential RNA polymerase binding protein, reveals a quasidomain-swapped dimeric structural architecture.
Proteins, 82:879-884, 2014

PubMed Abstract: Mycobacterium tuberculosis (Mtb) CarD is an essential transcriptional regulator that binds RNA polymerase and plays an important role in reprogramming transcription machinery under diverse stress conditions. Here, we report the crystal structure of CarD at 2.3 Å resolution, that represents the first structural description of CarD/CdnL-Like family of proteins. CarD adopts an overall bi-lobed structural architecture where N-terminal domain resembles 'tudor-like' domain and C-terminal domain adopts a novel five helical fold that lacks the predicted leucine zipper structural motif. The structure reveals dimeric state of CarD resulting from β-strand swapping between the N-terminal domains of each individual subunits. The structure provides crucial insights into the possible mode(s) of CarD/RNAP interactions.

PubMed: 24115125
DOI: 10.1002/prot.24419

実験手法

X-RAY DIFFRACTION (2.3 Å)