4IK4

High resolution structure of GCaMP3 at pH 5.0

Summary for 4IK4

Related4IK1 4IK3 4IK5 4IK8 4IK9
DescriptorRCaMP, Green fluorescent protein, CALCIUM ION (3 entities in total)
Functional Keywordscalcium indicator, mutants, fluorescent intensity, dimerization, beta barrel, calmodulin, fluorescent protein
Biological sourceEntacmaea quadricolor (Bubble-tip anemone)
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Total number of polymer chains1
Total molecular weight50704.7
Authors
Chen, Y.,Song, X.,Miao, L.,Zhu, Y.,Ji, G. (deposition date: 2012-12-25, release date: 2014-02-05, Last modification date: 2017-06-21)
Primary citation
Chen, Y.,Song, X.,Ye, S.,Miao, L.,Zhu, Y.,Zhang, R.G.,Ji, G.
Structural insight into enhanced calcium indicator GCaMP3 and GCaMPJ to promote further improvement.
Protein Cell, 4:299-309, 2013
PubMed: 23549615 (PDB entries with the same primary citation)
DOI: 10.1007/s13238-013-2103-4
MImport into Mendeley
Experimental method
X-RAY DIFFRACTION (2.01 Å)
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Structure validation

RfreeClashscoreRamachandran outliersSidechain outliersRSRZ outliers0.206302.6%1.0%MetricValuePercentile RanksWorseBetterPercentile relative to all X-ray structuresPercentile relative to X-ray structures of similar resolution

More Asymmetric unit images

Molmil generated image of 4ik4
no rotation
Molmil generated image of 4ik4
rotated about x axis by 90°
Molmil generated image of 4ik4
rotated about y axis by 90°

More Biological unit images

Molmil generated image of 4ik4
no rotation
Molmil generated image of 4ik4
rotated about x axis by 90°
Molmil generated image of 4ik4
rotated about y axis by 90°
(*)In the case of coarse surface representation, the asymmetric unit is shown as red ribbon representation.
Coordinate files for Biological unit (4ik4.pdb1.gz [274.56 KB])