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4IK1

High resolution structure of GCaMPJ at pH 8.5

Summary for 4IK1
Entry DOI10.2210/pdb4ik1/pdb
Related4IK3 4IK4 4IK5 4IK8 4IK9
DescriptorRCaMP, Green fluorescent protein, CALCIUM ION (3 entities in total)
Functional Keywordscalcium indicator, fluorescent intensity, dimerization, beta barrel, calmodulin, fluorescent protein
Biological sourceEntacmaea quadricolor (Bubble-tip anemone)
More
Total number of polymer chains1
Total formula weight50752.79
Authors
Chen, Y.,Song, X.,Miao, L.,Zhu, Y.,Ji, G. (deposition date: 2012-12-25, release date: 2014-02-05, Last modification date: 2017-06-21)
Primary citationChen, Y.,Song, X.,Ye, S.,Miao, L.,Zhu, Y.,Zhang, R.G.,Ji, G.
Structural insight into enhanced calcium indicator GCaMP3 and GCaMPJ to promote further improvement.
Protein Cell, 4:299-309, 2013
Cited by
PubMed Abstract: Genetically encoded Ca(2+) indicators (GECI) are important for the measurement of Ca(2+) in vivo. GCaMP2, a widely-used GECI, has recently been iteratively improved. Among the improved variants, GCaMP3 exhibits significantly better fluorescent intensity. In this study, we developed a new GECI called GCaMPJ and determined the crystal structures of GCaMP3 and GCaMPJ. GCaMPJ has a 1.5-fold increase in fluorescence and 1.3-fold increase in calcium affinity over GCaMP3. Upon Ca(2+) binding, GCaMP3 exhibits both monomeric and dimeric forms. The structural superposition of these two forms reveals the role of Arg-376 in improving monomer performance. However, GCaMPJ seldom forms dimers under conditions similar to GCaMP3. St ructural and mutagenesis studies on Tyr-380 confirmed its importance in blocking the cpEGFP β-barrel holes. Our study proposes an efficient tool for mapping Ca(2+) signals in intact organs to facilitate the further improvement of GCaMP sensors.
PubMed: 23549615
DOI: 10.1007/s13238-013-2103-4
PDB entries with the same primary citation
Experimental method
X-RAY DIFFRACTION (2 Å)
Structure validation

226707

數據於2024-10-30公開中

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