4III

Lambda-[Ru(TAP)2(11-Cl-dppz)] with a DNA decamer at atomic resolution

4III の概要

• エントリーDOI: 10.2210/pdb4iii/pdb
• 分子名称: 5'-D(*TP*CP*GP*GP*CP*GP*CP*CP*GP*A)-3', BARIUM ION, Ru(TAP)2(Cl-dppz) complex, ... (4 entities in total)
• 機能のキーワード: duplex, ruthenium, dna
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 3965.50

構造登録者

Hall, J.P.,Cardin, C.J. (登録日: 2012-12-20, 公開日: 2013-07-17, 最終更新日: 2023-11-08)

主引用文献

Hall, J.P.,Beer, H.,Buchner, K.,Cardin, D.J.,Cardin, C.J.
Preferred orientation in an angled intercalation site of a chloro-substituted Lambda-[Ru(TAP)2(dppz)]2+ complex bound to d(TCGGCGCCGA)2.
Philos Trans A Math Phys Eng Sci, 371:20120525-20120525, 2013

PubMed Abstract: The crystal structure of the ruthenium DNA 'light-switch' complex Λ-[Ru(TAP)2(11-Cl-dppz)](2+) (TAP=tetraazaphenanthrene, dppz=dipyrido[3,2-a':2',3'-c]phenazine) bound to the oligonucleotide duplex d(TCGGCGCCGA)2 is reported. The synthesis of the racemic ruthenium complex is described for the first time, and the racemate was used in this study. The crystal structure, at atomic resolution (1.0 Å), shows one ligand as a wedge in the minor groove, resulting in the 51(°) kinking of the double helix, as with the parent Λ-[Ru(TAP)2(dppz)](2+). Each complex binds to one duplex by intercalation of the dppz ligand and also by semi-intercalation of one of the orthogonal TAP ligands into a second symmetrically equivalent duplex. The 11-chloro substituent binds with the major component (66%) oriented with the 11-chloro substituent on the purine side of the terminal step of the duplex.

PubMed: 23776304
DOI: 10.1098/rsta.2012.0525

実験手法

X-RAY DIFFRACTION (1.02 Å)