4IF2

Structure of the phosphotriesterase from Mycobacterium tuberculosis

4IF2 の概要

• エントリーDOI: 10.2210/pdb4if2/pdb
• 分子名称: Phosphotriesterase homology protein, ZINC ION (3 entities in total)
• 機能のキーワード: double metal ions binding protein, enzymatic antidotes for organophosphates, hydrolase
• 由来する生物種: Mycobacterium tuberculosis
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 36103.65

構造登録者

Zhang, L.,Li, X.,Rao, Z.H. (登録日: 2012-12-13, 公開日: 2013-12-18, 最終更新日: 2022-08-24)

主引用文献

Zhang, L.,Wang, H.,Liu, X.,Zhou, W.,Rao, Z.
The crystal structure of the phosphotriesterase from M. tuberculosis, another member of phosphotriesterase-like lactonase family.
Biochem.Biophys.Res.Commun., 510:224-229, 2019

PubMed Abstract: Organophosphates (OPs) have been used widely as insecticides for protecting the agricultural crops from the pests. These compounds are highly toxic because they can cause the irreversible damage to human nervous system. Phosphotriesterases (PTEs), widely exist in many different kinds of bacteria, insects and mammals, can hydrolyze phosphotriesters (one major kind of OP) sufficiently. The phosphotriesterase-activities of PTEs are considered to derive from the lactonase-activities during the evolution, and phosphotriesterase-like lactonase family (PLL), is the closest protein family to PTE family based on protein-protein blast results. But members of PLL family exhibit higher lactonase activities than the phosphotriesterase activities, while the best substrates for PTEs are phosphotriesters. In this paper, the X-ray crystal structure of phosphotriesterase from M. tuberculosis (mPHP) was solved at a resolution of 2.3 Å. The structure reveals that the mPHP is a dimer with a typical distorted (β/α) barrel structure like other structures of PLL family and PTE family. The architecture of active pocket of mPHP coordinates with 2 metal ions which is also similar to other PLLs and PTEs. The activity assay proved the mPHP is biological active form and the Atomic Absorption Spectroscopy assay gave the evidence that the two metal ions bound to the active pocket were Zinc cations. The structural comparison between mPHP and other homologues concluded that the mPHP should belong to PLL family, not PTE family.

PubMed: 30704759
DOI: 10.1016/j.bbrc.2019.01.069

実験手法

X-RAY DIFFRACTION (2.27 Å)