4IDL

Low melting temperature Anti-Cholera Toxin Llama VHH domain

Summary for 4IDL

• Entry DOI: 10.2210/pdb4idl/pdb
• Descriptor: Single Domain Antibody VHH A9 (2 entities in total)
• Functional Keywords: v-type immunoglobin fold, antibody, cholera toxin, immune system, single domain antibody
• Biological source: Lama glama
• Total number of polymer chains: 1
• Total formula weight: 14895.43

Authors

Legler, P.M.,Lam, A.,Hol, W.G.J. (deposition date: 2012-12-12, release date: 2013-02-13, Last modification date: 2024-10-09)

Primary citation

Legler, P.M.,Zabetakis, D.,Anderson, G.P.,Lam, A.,Hol, W.G.,Goldman, E.R.
Structure of a low-melting-temperature anti-cholera toxin: llama V(H)H domain.
Acta Crystallogr.,Sect.F, 69:90-93, 2013

PubMed Abstract: Variable heavy domains derived from the heavy-chain-only antibodies found in camelids (V(H)H domains) are known for their thermal stability. Here, the structure of A9, an anti-cholera toxin V(H)H domain (K(d) = 77 ± 5 nM) that has an unusually low melting temperature of 319.9 ± 1.6 K, is reported. The CDR3 residues of A9 form a β-hairpin that is directed away from the former V(H)-V(L) interfacial surface, exposing hydrophobic residues to the solvent. A DALI structural similarity search showed that this CDR3 conformation is uncommon.

PubMed: 23385744
DOI: 10.1107/S1744309112050750

Experimental method

X-RAY DIFFRACTION (2.09 Å)