4IDL

Low melting temperature Anti-Cholera Toxin Llama VHH domain

4IDL の概要

• エントリーDOI: 10.2210/pdb4idl/pdb
• 分子名称: Single Domain Antibody VHH A9 (2 entities in total)
• 機能のキーワード: v-type immunoglobin fold, antibody, cholera toxin, immune system, single domain antibody
• 由来する生物種: Lama glama
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 14895.43

構造登録者

Legler, P.M.,Lam, A.,Hol, W.G.J. (登録日: 2012-12-12, 公開日: 2013-02-13, 最終更新日: 2024-10-09)

主引用文献

Legler, P.M.,Zabetakis, D.,Anderson, G.P.,Lam, A.,Hol, W.G.,Goldman, E.R.
Structure of a low-melting-temperature anti-cholera toxin: llama V(H)H domain.
Acta Crystallogr.,Sect.F, 69:90-93, 2013

PubMed Abstract: Variable heavy domains derived from the heavy-chain-only antibodies found in camelids (V(H)H domains) are known for their thermal stability. Here, the structure of A9, an anti-cholera toxin V(H)H domain (K(d) = 77 ± 5 nM) that has an unusually low melting temperature of 319.9 ± 1.6 K, is reported. The CDR3 residues of A9 form a β-hairpin that is directed away from the former V(H)-V(L) interfacial surface, exposing hydrophobic residues to the solvent. A DALI structural similarity search showed that this CDR3 conformation is uncommon.

PubMed: 23385744
DOI: 10.1107/S1744309112050750

実験手法

X-RAY DIFFRACTION (2.09 Å)