4ID8

The crystal structure of a [3Fe-4S] ferredoxin associated with CYP194A4 from R. palustris HaA2

4ID8 の概要

• エントリーDOI: 10.2210/pdb4id8/pdb
• 分子名称: Putative ferredoxin, FE3-S4 CLUSTER (3 entities in total)
• 機能のキーワード: 4fe-4s single cluster domain, electron transport
• 由来する生物種: Rhodopseudomonas palustris
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 7888.24

構造登録者

Zhou, W.H.,Zhang, T.,Zhang, A.L.,Bell, S.G.,Wong, L.-L. (登録日: 2012-12-11, 公開日: 2013-12-11, 最終更新日: 2023-09-20)

主引用文献

Zhang, T.,Zhang, A.,Bell, S.G.,Wong, L.L.,Zhou, W.
The structure of a novel electron-transfer ferredoxin from Rhodopseudomonas palustris HaA2 which contains a histidine residue in its iron-sulfur cluster-binding motif.
Acta Crystallogr.,Sect.D, 70:1453-1464, 2014

PubMed Abstract: Rhodopseudomonas palustris HaA2 contains a gene, RPB3630, encoding a ferredoxin, HaPuxC, with an atypical CXXHXXC(X)nCP iron-sulfur cluster-binding motif. The ferredoxin gene is associated with a cytochrome P450 (CYP) monooxygenase-encoding gene, CYP194A3, an arrangement which is conserved in several strains of bacteria. Similar ferredoxin genes are found in other bacteria, such as Mycobacterium tuberculosis, where they are also associated with CYP genes. The crystal structure of HaPuxC has been solved at 2.3 Å resolution. The overall fold of this [3Fe-4S] cluster-containing ferredoxin is similar to other [3Fe-4S] and [4Fe-4S] species, with the loop around the iron-sulfur cluster more closely resembling those of [3Fe-4S] ferredoxins. The side chain of His17 from the cluster-binding motif in HaPuxC points away from the vacant site of the cluster and interacts with Glu61 and one of the sulfide ions of the cluster. This is the first cytochrome P450 electron-transfer partner of this type to be structurally characterized and will provide a better understanding of the electron-transfer processes between these ferredoxins and their CYP enzymes.

PubMed: 24816113
DOI: 10.1107/S139900471400474X

実験手法

X-RAY DIFFRACTION (2.15 Å)