4IC1

Crystal structure of SSO0001

4IC1 の概要

• エントリーDOI: 10.2210/pdb4ic1/pdb
• 分子名称: Uncharacterized protein, MANGANESE (II) ION, IRON/SULFUR CLUSTER, ... (4 entities in total)
• 機能のキーワード: cas4, crispr, mcsg, exonuclease, psi-biology, structural genomics, unknown function, midwest center for structural genomics
• 由来する生物種: Sulfolobus solfataricus
• タンパク質・核酸の鎖数: 10
• 化学式量合計: 243137.89

構造登録者

Nocek, B.,Skarina, T.,Lemak, S.,Beloglazova, N.,Flick, R.,Brown, G.,Savchenko, A.,Joachimiak, A.,Yakunin, A.F.,Midwest Center for Structural Genomics (MCSG) (登録日: 2012-12-09, 公開日: 2013-01-16, 最終更新日: 2024-10-30)

主引用文献

Lemak, S.,Beloglazova, N.,Nocek, B.,Skarina, T.,Flick, R.,Brown, G.,Popovic, A.,Joachimiak, A.,Savchenko, A.,Yakunin, A.F.
Toroidal structure and DNA cleavage by the CRISPR-associated [4Fe-4S] cluster containing Cas4 nuclease SSO0001 from Sulfolobus solfataricus.
J.Am.Chem.Soc., 135:17476-17487, 2013

PubMed Abstract: Cas4 proteins, a core protein family associated with the microbial system of adaptive immunity CRISPR, are predicted to function in the adaptation step of the CRISPR mechanism. Here we show that the Cas4 protein SSO0001 from the archaeon Sulfolobus solfataricus has metal-dependent endonuclease and 5'→3' exonuclease activities against single-stranded DNA, as well as ATP-independent DNA unwinding activity toward double-stranded DNA. The crystal structure of SSO0001 revealed a decameric toroid formed by five dimers with each protomer containing one [4Fe-4S] cluster and one Mn(2+) ion bound in the active site located inside the internal tunnel. The conserved RecB motif and four Cys residues are important for DNA binding and cleavage activities, whereas DNA unwinding depends on several residues located near the [4Fe-4S] cluster. Our results suggest that Cas4 proteins might contribute to the addition of novel CRISPR spacers through the formation of 3'-DNA overhangs and to the degradation of foreign DNA.

PubMed: 24171432
DOI: 10.1021/ja408729b

実験手法

X-RAY DIFFRACTION (2.35 Å)