4IBN
Crystal structure of LC9-RNase H1, a type 1 RNase H with the type 2 active-site motif
Summary for 4IBN
| Entry DOI | 10.2210/pdb4ibn/pdb |
| Descriptor | Ribonuclease H (2 entities in total) |
| Functional Keywords | rnase h, ribonuclease h, hydrolase', hydrolase |
| Biological source | uncultured organism |
| Total number of polymer chains | 1 |
| Total formula weight | 23832.58 |
| Authors | Nguyen, T.-N.,You, D.-J.,Kanaya, E.,Koga, Y.,Kanaya, S. (deposition date: 2012-12-09, release date: 2013-03-20, Last modification date: 2023-11-08) |
| Primary citation | Nguyen, T.-N.,You, D.-J.,Kanaya, E.,Koga, Y.,Kanaya, S. Crystal structure of metagenome-derived LC9-RNase H1 with atypical DEDN active site motif Febs Lett., 587:1418-1423, 2013 Cited by PubMed Abstract: The crystal structure of metagenome-derived LC9-RNase H1 was determined. The structure-based mutational analyses indicated that the active site motif of LC9-RNase H1 is altered from DEDD to DEDN. In this motif, the location of the second glutamate residue is moved from αA-helix to β1-strand immediately next to the first aspartate residue, as in the active site of RNase H2. However, the structure and enzymatic properties of LC9-RNase H1 highly resemble those of RNase H1, instead of RNase H2. We propose that LC9-RNase H1 represents bacterial RNases H1 with an atypical DEDN active site motif, which are evolutionarily distinct from those with a typical DEDD active site motif. PubMed: 23523920DOI: 10.1016/j.febslet.2013.03.020 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (1.62 Å) |
Structure validation
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