4IBN

Crystal structure of LC9-RNase H1, a type 1 RNase H with the type 2 active-site motif

4IBN の概要

• エントリーDOI: 10.2210/pdb4ibn/pdb
• 分子名称: Ribonuclease H (2 entities in total)
• 機能のキーワード: rnase h, ribonuclease h, hydrolase', hydrolase
• 由来する生物種: uncultured organism
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 23832.58

構造登録者

Nguyen, T.-N.,You, D.-J.,Kanaya, E.,Koga, Y.,Kanaya, S. (登録日: 2012-12-09, 公開日: 2013-03-20, 最終更新日: 2023-11-08)

主引用文献

Nguyen, T.-N.,You, D.-J.,Kanaya, E.,Koga, Y.,Kanaya, S.
Crystal structure of metagenome-derived LC9-RNase H1 with atypical DEDN active site motif
Febs Lett., 587:1418-1423, 2013

PubMed Abstract: The crystal structure of metagenome-derived LC9-RNase H1 was determined. The structure-based mutational analyses indicated that the active site motif of LC9-RNase H1 is altered from DEDD to DEDN. In this motif, the location of the second glutamate residue is moved from αA-helix to β1-strand immediately next to the first aspartate residue, as in the active site of RNase H2. However, the structure and enzymatic properties of LC9-RNase H1 highly resemble those of RNase H1, instead of RNase H2. We propose that LC9-RNase H1 represents bacterial RNases H1 with an atypical DEDN active site motif, which are evolutionarily distinct from those with a typical DEDD active site motif.

PubMed: 23523920
DOI: 10.1016/j.febslet.2013.03.020

実験手法

X-RAY DIFFRACTION (1.62 Å)