4I8T

C.Esp1396I bound to a 19 base pair DNA duplex

4I8T の概要

• エントリーDOI: 10.2210/pdb4i8t/pdb
• 関連するPDBエントリー: 3CLC 3FYA 3G5G 3S8Q 3UFD 4F8D 4FBI 4FN3 4I6R 4I6T 4I6U
• 分子名称: Regulatory protein, DNA (5'-D(P*TP*GP*TP*TP*GP*AP*CP*TP*AP*TP*AP*AP*TP*CP*AP*CP*AP*CP*A)-3'), DNA (5'-D(P*TP*GP*TP*GP*TP*GP*AP*TP*TP*AP*TP*AP*GP*TP*CP*AP*AP*CP*A)-3') (3 entities in total)
• 機能のキーワード: restriction-modification, helix-turn-helix, transcriptional regulator, dna, transcription-dna complex, transcription/dna
• 由来する生物種: Enterobacter sp.; 詳細
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 30688.95

構造登録者

Martin, R.N.A.,McGeehan, J.E.,Kneale, G.G. (登録日: 2012-12-04, 公開日: 2013-09-11, 最終更新日: 2023-09-20)

主引用文献

Martin, R.N.,McGeehan, J.E.,Ball, N.J.,Streeter, S.D.,Thresh, S.J.,Kneale, G.G.
Structural analysis of DNA-protein complexes regulating the restriction-modification system Esp1396I.
Acta Crystallogr.,Sect.F, 69:962-966, 2013

PubMed Abstract: The controller protein of the type II restriction-modification (RM) system Esp1396I binds to three distinct DNA operator sequences upstream of the methyltransferase and endonuclease genes in order to regulate their expression. Previous biophysical and crystallographic studies have shown molecular details of how the controller protein binds to the operator sites with very different affinities. Here, two protein-DNA co-crystal structures containing portions of unbound DNA from native operator sites are reported. The DNA in both complexes shows significant distortion in the region between the conserved symmetric sequences, similar to that of a DNA duplex when bound by the controller protein (C-protein), indicating that the naked DNA has an intrinsic tendency to bend when not bound to the C-protein. Moreover, the width of the major groove of the DNA adjacent to a bound C-protein dimer is observed to be significantly increased, supporting the idea that this DNA distortion contributes to the substantial cooperativity found when a second C-protein dimer binds to the operator to form the tetrameric repression complex.

PubMed: 23989141
DOI: 10.1107/S174430911302126X

実験手法

X-RAY DIFFRACTION (3 Å)