4I7A

GrpN pentameric microcompartment shell protein from Rhodospirillum rubrum

4I7A の概要

• エントリーDOI: 10.2210/pdb4i7a/pdb
• 分子名称: Ethanolamine utilization protein EutN/carboxysome structural protein Ccml, CHLORIDE ION (3 entities in total)
• 機能のキーワード: microcompartments, ccml, eutn, glycyl radical propanediol microcompartment, structural protein
• 由来する生物種: Rhodospirillum rubrum
• タンパク質・核酸の鎖数: 5
• 化学式量合計: 49507.12

構造登録者

Wheatley, N.M.,Gidaniyan, S.D.,Cascio, D.,Yeates, T.O. (登録日: 2012-11-30, 公開日: 2013-05-01, 最終更新日: 2023-09-20)

主引用文献

Wheatley, N.M.,Gidaniyan, S.D.,Liu, Y.,Cascio, D.,Yeates, T.O.
Bacterial microcompartment shells of diverse functional types possess pentameric vertex proteins.
Protein Sci., 22:660-665, 2013

PubMed Abstract: Bacterial microcompartments (MCPs) are large proteinaceous structures comprised of a roughly icosahedral shell and a series of encapsulated enzymes. MCPs carrying out three different metabolic functions have been characterized in some detail, while gene expression and bioinformatics studies have implicated other types, including one believed to perform glycyl radical-based metabolism of 1,2-propanediol (Grp). Here we report the crystal structure of a protein (GrpN), which is presumed to be part of the shell of a Grp-type MCP in Rhodospirillum rubrum F11. GrpN is homologous to a family of proteins (EutN/PduN/CcmL/CsoS4) whose members have been implicated in forming the vertices of MCP shells. Consistent with that notion, the crystal structure of GrpN revealed a pentameric assembly. That observation revived an outstanding question about the oligomeric state of this protein family: pentameric forms (for CcmL and CsoS4A) and a hexameric form (for EutN) had both been observed in previous crystal structures. To clarify these confounding observations, we revisited the case of EutN. We developed a molecular biology-based method for accurately determining the number of subunits in homo-oligomeric proteins, and found unequivocally that EutN is a pentamer in solution. Based on these convergent findings, we propose the name bacterial microcompartment vertex for this special family of MCP shell proteins.

PubMed: 23456886
DOI: 10.1002/pro.2246

実験手法

X-RAY DIFFRACTION (3.2 Å)