4I6O

Crystal structure of chemically synthesized human anaphylatoxin C3a

4I6O の概要

• エントリーDOI: 10.2210/pdb4i6o/pdb
• 分子名称: Complement C3, (4S)-2-METHYL-2,4-PENTANEDIOL (3 entities in total)
• 機能のキーワード: disulfide-rich, alpha-helical bundle, drumstick, complement system, third component of complement (c3), local inflammatory process mediator, smooth muscle contraction induction, vascular permeability increase, mast cell histamine release, c3a receptor, gpcr, extracellular, blood plasma, immune system
• 由来する生物種: Homo sapiens (human)
• 細胞内の位置: Secreted: P01024
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 9232.91

構造登録者

Wang, C.I.A.,Ghassemian, A.,Collins, B.,Lewis, R.J.,Alewood, P.F.,Durek, T. (登録日: 2012-11-29, 公開日: 2013-02-27, 最終更新日: 2024-10-09)

主引用文献

Ghassemian, A.,Wang, C.I.,Yau, M.K.,Reid, R.C.,Lewis, R.J.,Fairlie, D.P.,Alewood, P.F.,Durek, T.
Efficient chemical synthesis of human complement protein C3a.
Chem.Commun.(Camb.), 49:2356-2358, 2013

PubMed Abstract: We report the total chemical synthesis of human C3a by one-pot native chemical ligation of three unprotected peptide segments, followed by efficient in vitro folding that yielded the anaphylatoxin C3a in high yield and excellent purity. Synthetic C3a was fully active and its crystal structure at 2.1 Å resolution showed 3 helices and a C-terminal turn motif.

PubMed: 23407800
DOI: 10.1039/c3cc40537k

実験手法

X-RAY DIFFRACTION (2.14 Å)