4I3J

Structures of PR1 intermediate of photoactive yellow protein E46Q mutant from time-resolved laue crystallography collected AT 14ID APS

4I3J の概要

• エントリーDOI: 10.2210/pdb4i3j/pdb
• 関連するPDBエントリー: 1TS0 1TS6 1TS7 1TS8 3VE3 3VE4 4HY8 4I38 4I39 4I3A 4I3I
• 分子名称: Photoactive yellow protein, 4'-HYDROXYCINNAMIC ACID (2 entities in total)
• 機能のキーワード: photoreceptor, chromophore, photoreceptor protein, receptor, sensory transduction, luminescent protein
• 由来する生物種: Halorhodospira halophila
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 14051.75

構造登録者

Jung, Y.O.,Lee, J.H.,Kim, J.,Schmidt, M.,Vukica, S.,Moffat, K.,Ihee, H. (登録日: 2012-11-26, 公開日: 2013-03-20, 最終更新日: 2023-11-15)

主引用文献

Jung, Y.O.,Lee, J.H.,Kim, J.,Schmidt, M.,Moffat, K.,Srajer, V.,Ihee, H.
Volume-conserving trans-cis isomerization pathways in photoactive yellow protein visualized by picosecond X-ray crystallography
NAT.CHEM., 5:212-220, 2013

PubMed Abstract: Trans-to-cis isomerization, the key reaction in photoactive proteins, usually cannot occur through the standard one-bond-flip mechanism. Owing to spatial constraints imposed by a protein environment, isomerization probably proceeds through a volume-conserving mechanism in which highly choreographed atomic motions are expected, the details of which have not yet been observed directly. Here we employ time-resolved X-ray crystallography to visualize structurally the isomerization of the p-coumaric acid chromophore in photoactive yellow protein with a time resolution of 100 ps and a spatial resolution of 1.6 Å. The structure of the earliest intermediate (I(T)) resembles a highly strained transition state in which the torsion angle is located halfway between the trans- and cis-isomers. The reaction trajectory of I(T) bifurcates into two structurally distinct cis intermediates via hula-twist and bicycle-pedal pathways. The bifurcating reaction pathways can be controlled by weakening the hydrogen bond between the chromophore and an adjacent residue through E46Q mutation, which switches off the bicycle-pedal pathway.

PubMed: 23422563
DOI: 10.1038/nchem.1565

実験手法

X-RAY DIFFRACTION (1.6 Å)