4HZO

The Structure of the Bifunctional Acetyltransferase/Decarboxylase LnmK from the Leinamycin Biosynthetic Pathway Revealing Novel Activity for a Double Hot Dog Fold

4HZO の概要

• エントリーDOI: 10.2210/pdb4hzo/pdb
• 関連するPDBエントリー: 4HZN
• 分子名称: Bifunctional methylmalonyl-CoA:ACP acyltransferase/decarboxylase, CHLORIDE ION, COENZYME A, ... (4 entities in total)
• 機能のキーワード: structural genomics, protein structure initiative, enzyme discovery for natural product biosynthesis, natpro, psi-biology, double hot dog fold, acyl carrier protein - lnml, transferase, lyase
• 由来する生物種: Streptomyces atroolivaceus
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 36082.27

構造登録者

Lohman, J.R.,Bingman, C.A.,Phillips Jr., G.N.,Shen, B.,Enzyme Discovery for Natural Product Biosynthesis (NatPro) (登録日: 2012-11-15, 公開日: 2013-01-30, 最終更新日: 2024-02-28)

主引用文献

Lohman, J.R.,Bingman, C.A.,Phillips, G.N.,Shen, B.
Structure of the Bifunctional Acyltransferase/Decarboxylase LnmK from the Leinamycin Biosynthetic Pathway Revealing Novel Activity for a Double-Hot-Dog Fold.
Biochemistry, 52:902-911, 2013

PubMed Abstract: The β-branched C3 unit in leinamycin biosynthesis is installed by a set of four proteins, LnmFKLM. In vitro biochemical investigation confirmed that LnmK is a bifunctional acyltransferase/decarboxylase (AT/DC) that catalyzes first self-acylation using methylmalonyl-CoA as a substrate and subsequently transacylation of the methylmalonyl group to the phosphopantetheinyl group of the LnmL acyl carrier protein [Liu, T., Huang, Y., and Shen, B. (2009) J. Am. Chem. Soc. 131, 6900-6901]. LnmK shows no sequence homology to proteins of known function, representing a new family of AT/DC enzymes. Here we report the X-ray structure of LnmK. LnmK is homodimer with each of the monomers adopting a double-hot-dog fold. Cocrystallization of LnmK with methylmalonyl-CoA revealed an active site tunnel terminated by residues from the dimer interface. In contrast to canonical AT and ketosynthase enzymes that employ Ser or Cys as an active site residue, none of these residues are found in the vicinity of the LnmK active site. Instead, three tyrosines were identified, one of which, Tyr62, was established, by site-directed mutagenesis, to be the most likely active site residue for the AT activity of LnmK. LnmK represents the first AT enzyme that employs a Tyr as an active site residue and the first member of the family of double-hot-dog fold enzymes that displays an AT activity known to date. The LnmK structure sets the stage for probing of the DC activity of LnmK through site-directed mutagenesis. These findings highlight natural product biosynthetic machinery as a rich source of novel enzyme activities, mechanisms, and structures.

PubMed: 23320975
DOI: 10.1021/bi301652y

実験手法

X-RAY DIFFRACTION (1.76 Å)