4HWC

Structure of ATBAG1

4HWC の概要

• エントリーDOI: 10.2210/pdb4hwc/pdb
• 関連するPDBエントリー: 4HWD 4HWF 4HWH 4HWI
• 分子名称: BAG family molecular chaperone regulator 1, CHLORIDE ION (3 entities in total)
• 機能のキーワード: three helix bundle, co-chaperone, apoptosis
• 由来する生物種: Arabidopsis thaliana (mouse-ear cress)
• タンパク質・核酸の鎖数: 6
• 化学式量合計: 58429.34

構造登録者

Shen, Y.,Fang, S. (登録日: 2012-11-07, 公開日: 2013-05-29, 最終更新日: 2024-02-28)

主引用文献

Fang, S.,Li, L.,Cui, B.,Men, S.,Shen, Y.,Yang, X.
Structural insight into plant programmed cell death mediated by BAG proteins in Arabidopsis thaliana.
Acta Crystallogr.,Sect.D, 69:934-945, 2013

PubMed Abstract: The recently identified plant Bcl-2-associated athanogene (BAG) family plays an extensive role in plant programmed cell death (PCD) processes ranging from growth and development to stress responses and even cell death. In the Arabidopsis thaliana BAG (AtBAG) protein family, four members (AtBAG1-4) have a domain organization similar to that of mammalian BAG proteins. Here, crystal structures of the BAG domains (BDs) of AtBAG1-4 have been determined; they have high homology and adopt a structure comprising three short parallel α-helices, similar to some mammalian BAG proteins. The crystal structure of a complex of the AtBAG1 ubiquitin-like domain and BAG domain (UBD) with the Hsc70 nucleotide-binding domain (NBD) was also determined. The binding of the AtBAG1 BD to the Hsc70 NBD induces conformational change of the Hsc70 NBD to the open state and reduces the affinity of the NBD for ADP. In vivo studies showed that bag2-1 mutant plants are larger than wild-type plants when growing under normal conditions, indicating that the AtBAG proteins might regulate plant PCD and confer tolerance to stresses in plants. These structural and functional analyses indicate that the AtBAG proteins function as nucleotide-exchange factors for Hsp70/Hsc70 in A. thaliana and that the mechanism of regulation of chaperone-mediated protein folding is conserved in plants.

PubMed: 23695238
DOI: 10.1107/S0907444913003624

実験手法

X-RAY DIFFRACTION (1.798 Å)