4HTA

The structure of the karrikin insensitive (KAI2) protein in Arabidopsis thaliana

4HTA の概要

• エントリーDOI: 10.2210/pdb4hta/pdb
• 関連するPDBエントリー: 4HRX 4HRY
• 分子名称: Hydrolase, alpha/beta fold family protein, GLYCEROL, PHOSPHATE ION, ... (4 entities in total)
• 機能のキーワード: alpha/beta hydrolase, signaling protein, hydrolase
• 由来する生物種: Arabidopsis thaliana (mouse-ear cress, thale-cress)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 32527.74

構造登録者

Bythell-Douglas, R.,Waters, M.T.,Scaffidi, A.,Flematti, G.R.,Smith, S.M.,Bond, C.S. (登録日: 2012-11-01, 公開日: 2013-02-27, 最終更新日: 2024-03-20)

主引用文献

Bythell-Douglas, R.,Waters, M.T.,Scaffidi, A.,Flematti, G.R.,Smith, S.M.,Bond, C.S.
The Structure of the Karrikin-Insensitive Protein (KAI2) in Arabidopsis thaliana
Plos One, 8:e54758-e54758, 2013

PubMed Abstract: KARRIKIN INSENSITIVE 2 (KAI2) is an α/β hydrolase involved in seed germination and seedling development. It is essential for plant responses to karrikins, a class of butenolide compounds derived from burnt plant material that are structurally similar to strigolactone plant hormones. The mechanistic basis for the function of KAI2 in plant development remains unclear. We have determined the crystal structure of Arabidopsis thaliana KAI2 in space groups P2(1) 2(1) 2(1) (a =63.57 Å, b =66.26 Å, c =78.25 Å) and P2(1) (a =50.20 Å, b =56.04 Å, c =52.43 Å, β =116.12°) to 1.55 and 2.11 Å respectively. The catalytic residues are positioned within a large hydrophobic pocket similar to that of DAD2, a protein required for strigolactone response in Petunia hybrida. KAI2 possesses a second solvent-accessible pocket, adjacent to the active site cavity, which offers the possibility of allosteric regulation. The structure of KAI2 is consistent with its designation as a serine hydrolase, as well as previous data implicating the protein in karrikin and strigolactone signalling.

PubMed: 23349965
DOI: 10.1371/journal.pone.0054758

実験手法

X-RAY DIFFRACTION (1.901 Å)