4HT6

The Structure of a Yeast Dynein Dyn2-Pac11 Complex and Effect on Single Molecule Dynein Motor Activity

4HT6 の概要

• エントリーDOI: 10.2210/pdb4ht6/pdb
• 分子名称: Dynein light chain 1, cytoplasmic, WD repeat-containing protein PAC11 (3 entities in total)
• 機能のキーワード: dimerization, dynein, intermediate chain, light chain, dynein intermediate chain dynein heavy chain, motor protein
• 由来する生物種: Saccharomyces cerevisiae (Baker's yeast); 詳細
• 細胞内の位置: Cytoplasm, cytoskeleton: Q02647; Cytoplasm, cytoskeleton (Potential): P40960
• タンパク質・核酸の鎖数: 6
• 化学式量合計: 36452.44

構造登録者

Slep, K.C.,Romes, E.R. (登録日: 2012-10-31, 公開日: 2013-09-18, 最終更新日: 2023-09-20)

主引用文献

Rao, L.,Romes, E.M.,Nicholas, M.P.,Brenner, S.,Tripathy, A.,Gennerich, A.,Slep, K.C.
The yeast dynein Dyn2-Pac11 complex is a dynein dimerization/processivity factor: structural and single-molecule characterization.
Mol Biol Cell, 24:2362-2377, 2013

PubMed Abstract: Cytoplasmic dynein is the major microtubule minus end-directed motor. Although studies have probed the mechanism of the C-terminal motor domain, if and how dynein's N-terminal tail and the accessory chains it binds regulate motor activity remain to be determined. Here, we investigate the structure and function of the Saccharomyces cerevisiae dynein light (Dyn2) and intermediate (Pac11) chains in dynein heavy chain (Dyn1) movement. We present the crystal structure of a Dyn2-Pac11 complex, showing Dyn2-mediated Pac11 dimerization. To determine the molecular effects of Dyn2 and Pac11 on Dyn1 function, we generated dyn2Δ and dyn2Δpac11Δ strains and analyzed Dyn1 single-molecule motor activity. We find that the Dyn2-Pac11 complex promotes Dyn1 homodimerization and potentiates processivity. The absence of Dyn2 and Pac11 yields motors with decreased velocity, dramatically reduced processivity, increased monomerization, aggregation, and immobility as determined by single-molecule measurements. Deleting dyn2 significantly reduces Pac11-Dyn1 complex formation, yielding Dyn1 motors with activity similar to Dyn1 from the dyn2Δpac11Δ strain. Of interest, motor phenotypes resulting from Dyn2-Pac11 complex depletion bear similarity to a point mutation in the mammalian dynein N-terminal tail (Loa), highlighting this region as a conserved, regulatory motor element.

PubMed: 23761070
DOI: 10.1091/mbc.E13-03-0166

実験手法

X-RAY DIFFRACTION (1.9 Å)