4HST

Crystal structure of a double mutant of a class III engineered cephalosporin acylase

4HST の概要

• エントリーDOI: 10.2210/pdb4hst/pdb
• 関連するPDBエントリー: 4HSR
• 分子名称: glutaryl-7-aminocephalosporanic acid acylase alpha chain, glutaryl-7-aminocephalosporanic acid acylase beta chain, 5,5-dihydroxy-L-norvaline, ... (4 entities in total)
• 機能のキーワード: protein engineering, substrate specificity, transition state analogue, n-terminal hydrolase, hydrolase
• 由来する生物種: Pseudomonas; 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 84231.23

構造登録者

Vrielink, A.,Golden, E.,Patterson, R.,Tie, W.J.,Anandan, A.,Flematti, G.,Molla, G.,Rosini, E.,Pollegioni, L. (登録日: 2012-10-30, 公開日: 2013-02-27, 最終更新日: 2024-02-28)

• 主引用文献: Golden, E.,Paterson, R.,Tie, W.J.,Anandan, A.,Flematti, G.,Molla, G.,Rosini, E.,Pollegioni, L.,Vrielink, A.; Structure of a class III engineered cephalosporin acylase: comparisons with class I acylase and implications for differences in substrate specificity and catalytic activity.; Biochem.J., 451:217-226, 2013; PubMed: 23373797; DOI: 10.1042/BJ20121715

実験手法

X-RAY DIFFRACTION (1.571 Å)