4HQM

The crystal structure of QsrR-menadione complex

4HQM の概要

• エントリーDOI: 10.2210/pdb4hqm/pdb
• 分子名称: QsrR protein, 2-methylnaphthalene-1,4-diol (2 entities in total)
• 機能のキーワード: menadione-modified protein, dna, transcription regulator
• 由来する生物種: Staphylococcus aureus
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 26492.33

構造登録者

Ji, Q.,Zhang, L.,Jones, M.B.,Sun, F.,Deng, X.,Liang, H.,Brugarolas, P.,Gao, N.,Peterson, S.N.,Lan, L.,Bae, T.,He, C. (登録日: 2012-10-25, 公開日: 2013-03-06, 最終更新日: 2024-11-20)

主引用文献

Ji, Q.,Zhang, L.,Jones, M.B.,Sun, F.,Deng, X.,Liang, H.,Cho, H.,Brugarolas, P.,Gao, Y.N.,Peterson, S.N.,Lan, L.,Bae, T.,He, C.
Molecular mechanism of quinone signaling mediated through S-quinonization of a YodB family repressor QsrR.
Proc.Natl.Acad.Sci.USA, 110:5010-5015, 2013

PubMed Abstract: Quinone molecules are intracellular electron-transport carriers, as well as critical intra- and extracellular signals. However, transcriptional regulation of quinone signaling and its molecular basis are poorly understood. Here, we identify a thiol-stress-sensing regulator YodB family transcriptional regulator as a central component of quinone stress response of Staphylococcus aureus, which we have termed the quinone-sensing and response repressor (QsrR). We also identify and confirm an unprecedented quinone-sensing mechanism based on the S-quinonization of the essential residue Cys-5. Structural characterizations of the QsrR-DNA and QsrR-menadione complexes further reveal that the covalent association of menadione directly leads to the release of QsrR from operator DNA following a 10° rigid-body rotation as well as a 9-Å elongation between the dimeric subunits. The molecular level characterization of this quinone-sensing transcriptional regulator provides critical insights into quinone-mediated gene regulation in human pathogens.

PubMed: 23479646
DOI: 10.1073/pnas.1219446110

実験手法

X-RAY DIFFRACTION (2.55 Å)