4HQ9

Crystal structure of a green-to-red photoconvertible DRONPA, pcDRONPA in the green-off-state

Summary for 4HQ9

• Entry DOI: 10.2210/pdb4hq9/pdb
• Related: 4HQ8 4HQC
• Descriptor: Fluorescent protein Dronpa, DI(HYDROXYETHYL)ETHER, TRIETHYLENE GLYCOL, ... (5 entities in total)
• Functional Keywords: green-to-red fluorescent protein, dronpa, beta-barrel, fluorescent protein
• Biological source: Echinophyllia
• Total number of polymer chains: 6
• Total formula weight: 177562.09

Authors

Nguyen Bich, N.,Van Meervelt, L. (deposition date: 2012-10-25, release date: 2013-11-13, Last modification date: 2024-10-30)

Primary citation

Moeyaert, B.,Nguyen Bich, N.,De Zitter, E.,Rocha, S.,Clays, K.,Mizuno, H.,van Meervelt, L.,Hofkens, J.,Dedecker, P.
Green-to-red photoconvertible Dronpa mutant for multimodal super-resolution fluorescence microscopy
ACS Nano, 8:1664-1673, 2014

PubMed Abstract: Advanced imaging techniques crucially depend on the labels used. In this work, we present the structure-guided design of a fluorescent protein that displays both reversibly photochromic and green-to-red photoconversion behavior. We first designed ffDronpa, a mutant of the photochromic fluorescent protein Dronpa that matures up to three times faster while retaining its interesting photochromic features. Using a combined evolutionary and structure-driven rational design strategy, we developed a green-to-red photoconvertible ffDronpa mutant, called pcDronpa, and explored different optimization strategies that resulted in its improved version, pcDronpa2. This fluorescent probe combines a high brightness with low photobleaching and photoblinking. We herein show that, despite its tetrameric nature, pcDronpa2 allows for multimodal subdiffraction imaging by sequentially imaging a given sample using both super-resolution fluctuation imaging and localization microscopy.

PubMed: 24410188
DOI: 10.1021/nn4060144

Experimental method

X-RAY DIFFRACTION (2.07 Å)