4HPP

Crystal structure of novel glutamine synthase homolog

4HPP の概要

• エントリーDOI: 10.2210/pdb4hpp/pdb
• 分子名称: Probable glutamine synthetase, MAGNESIUM ION, CALCIUM ION, ... (5 entities in total)
• 機能のキーワード: glutamine synthase homolog, glutamate, polyamine, ligase
• 由来する生物種: Pseudomonas aeruginosa
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 48165.97

構造登録者

Ladner, J.E.,Atanasova, V.,Dolezelova, Z.,Parsons, J.F. (登録日: 2012-10-24, 公開日: 2012-12-26, 最終更新日: 2023-09-20)

主引用文献

Ladner, J.E.,Atanasova, V.,Dolezelova, Z.,Parsons, J.F.
Structure and Activity of PA5508, a Hexameric Glutamine Synthetase Homologue.
Biochemistry, 51:10121-10123, 2012

PubMed Abstract: The structure of PA5508 from Pseudomonas aeruginosa, a glutamine synthetase (GS) homologue, has been determined at 2.5 Å. Surprisingly, PA5508 forms single hexameric rings rather than the stacked double rings that are characteristic of GS. The C-terminal helical thong motif that links GS rings is present in PA5508; however, it is folded back toward the core of its own polypeptide, preventing it from interacting with a second ring. Interestingly, PA5508 displays a clear preference for aromatic amine substrates. Unique aspects of the structure illustrate how the enzyme is able to catalyze reactions involving bulky amines rather than ammonia.

PubMed: 23234431
DOI: 10.1021/bi3014856

実験手法

X-RAY DIFFRACTION (2.5 Å)