4HPH

The crystal structure of isomaltulose synthase mutant E295Q from Erwinia rhapontici NX5 in complex with its natural substrate sucrose

4HPH の概要

• エントリーDOI: 10.2210/pdb4hph/pdb
• 関連するPDBエントリー: 4HOW 4HOX 4HOZ 4HP5
• 関連するBIRD辞書のPRD_ID: PRD_900003
• 分子名称: Sucrose isomerase, beta-D-fructofuranose-(2-1)-alpha-D-glucopyranose, CALCIUM ION, ... (5 entities in total)
• 機能のキーワード: tim barrel, isomerase, hydrolase, calcium binding
• 由来する生物種: Erwinia rhapontici
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 66304.66

構造登録者

Xu, Z.,Li, S.,Xu, H.,Zhou, J. (登録日: 2012-10-23, 公開日: 2013-10-16, 最終更新日: 2023-11-08)

主引用文献

Xu, Z.,Li, S.,Li, J.,Li, Y.,Feng, X.,Wang, R.,Xu, H.,Zhou, J.
The Structural Basis of Erwinia rhapontici Isomaltulose Synthase
Plos One, 8:e74788-e74788, 2013

PubMed Abstract: Sucrose isomerase NX-5 from Erwiniarhapontici efficiently catalyzes the isomerization of sucrose to isomaltulose (main product) and trehalulose (by-product). To investigate the molecular mechanism controlling sucrose isomer formation, we determined the crystal structures of native NX-5 and its mutant complexes E295Q/sucrose and D241A/glucose at 1.70 Å, 1.70 Å and 2.00 Å, respectively. The overall structure and active site architecture of NX-5 resemble those of other reported sucrose isomerases. Strikingly, the substrate binding mode of NX-5 is also similar to that of trehalulose synthase from Pseudomonasmesoacidophila MX-45 (MutB). Detailed structural analysis revealed the catalytic RXDRX motif and the adjacent 10-residue loop of NX-5 and isomaltulose synthase PalI from Klebsiella sp. LX3 adopt a distinct orientation from those of trehalulose synthases. Mutations of the loop region of NX-5 resulted in significant changes of the product ratio between isomaltulose and trehalulose. The molecular dynamics simulation data supported the product specificity of NX-5 towards isomaltulose and the role of the loop(330-339) in NX-5 catalysis. This work should prove useful for the engineering of sucrose isomerase for industrial carbohydrate biotransformations.

PubMed: 24069347
DOI: 10.1371/journal.pone.0074788

実験手法

X-RAY DIFFRACTION (1.7 Å)