4HK1

Crystal Structure of PCNA from Drosophila melanogaster

Summary for 4HK1

• Entry DOI: 10.2210/pdb4hk1/pdb
• Descriptor: Proliferating cell nuclear antigen (2 entities in total)
• Functional Keywords: dna binding protein, the sliding clamps family (beta clamps), dna binding, dna replication, dna repair, cell cycle control, cell cycle
• Biological source: Drosophila melanogaster (Fruit fly)
• Cellular location: Nucleus: P17917
• Total number of polymer chains: 1
• Total formula weight: 29127.27

Authors

Wang, K.,Shi, Z.B.,Zhou, Z.C. (deposition date: 2012-10-14, release date: 2013-04-10, Last modification date: 2023-09-20)

Primary citation

Wang, K.,Shi, Z.,Zhang, M.,Cheng, D.
Structure of PCNA from Drosophila melanogaster.
Acta Crystallogr.,Sect.F, 69:387-392, 2013

PubMed Abstract: Proliferating cell nuclear antigen (PCNA) plays essential roles in DNA replication, DNA repair, cell-cycle regulation and chromatin metabolism. The PCNA from Drosophila melanogaster (DmPCNA) was purified and crystallized. The crystal of DmPCNA diffracted to 2.0 Å resolution and belonged to space group H3, with unit-cell parameters a = b = 151.16, c = 38.28 Å. The structure of DmPCNA was determined by molecular replacement. DmPCNA forms a symmetric homotrimer in a head-to-tail manner. An interdomain connector loop (IDCL) links the N- and C-terminal domains. Additionally, the N-terminal and C-terminal domains contact each other through hydrophobic associations. Compared with human PCNA, the IDCL of DmPCNA has conformational changes, which may explain their difference in function. This work provides a structural basis for further functional and evolutionary studies of PCNA.

PubMed: 23545643
DOI: 10.1107/S1744309113004971

Experimental method

X-RAY DIFFRACTION (2.001 Å)