4HK1
Crystal Structure of PCNA from Drosophila melanogaster
Summary for 4HK1
| Entry DOI | 10.2210/pdb4hk1/pdb |
| Descriptor | Proliferating cell nuclear antigen (2 entities in total) |
| Functional Keywords | dna binding protein, the sliding clamps family (beta clamps), dna binding, dna replication, dna repair, cell cycle control, cell cycle |
| Biological source | Drosophila melanogaster (Fruit fly) |
| Cellular location | Nucleus: P17917 |
| Total number of polymer chains | 1 |
| Total formula weight | 29127.27 |
| Authors | Wang, K.,Shi, Z.B.,Zhou, Z.C. (deposition date: 2012-10-14, release date: 2013-04-10, Last modification date: 2023-09-20) |
| Primary citation | Wang, K.,Shi, Z.,Zhang, M.,Cheng, D. Structure of PCNA from Drosophila melanogaster. Acta Crystallogr.,Sect.F, 69:387-392, 2013 Cited by PubMed Abstract: Proliferating cell nuclear antigen (PCNA) plays essential roles in DNA replication, DNA repair, cell-cycle regulation and chromatin metabolism. The PCNA from Drosophila melanogaster (DmPCNA) was purified and crystallized. The crystal of DmPCNA diffracted to 2.0 Å resolution and belonged to space group H3, with unit-cell parameters a = b = 151.16, c = 38.28 Å. The structure of DmPCNA was determined by molecular replacement. DmPCNA forms a symmetric homotrimer in a head-to-tail manner. An interdomain connector loop (IDCL) links the N- and C-terminal domains. Additionally, the N-terminal and C-terminal domains contact each other through hydrophobic associations. Compared with human PCNA, the IDCL of DmPCNA has conformational changes, which may explain their difference in function. This work provides a structural basis for further functional and evolutionary studies of PCNA. PubMed: 23545643DOI: 10.1107/S1744309113004971 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (2.001 Å) |
Structure validation
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