4HJI

Structure of the CooA pilin subunit from enterotoxigenic Escherichia coli

4HJI の概要

• エントリーDOI: 10.2210/pdb4hji/pdb
• 分子名称: CS1 fimbrial subunit A, IMIDAZOLE, SODIUM ION, ... (4 entities in total)
• 機能のキーワード: cs1 pilus, colonization factor, pilin, chaperone-usher family, bacterial surface, cell adhesion
• 由来する生物種: Escherichia coli
• 細胞内の位置: Fimbrium: P0ABW7
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 36582.57

構造登録者

Kolappan, S.,Zong, Z.,Craig, L. (登録日: 2012-10-12, 公開日: 2012-12-19, 最終更新日: 2023-09-20)

主引用文献

Galkin, V.E.,Kolappan, S.,Ng, D.,Zong, Z.,Li, J.,Yu, X.,Egelman, E.H.,Craig, L.
The Structure of the CS1 Pilus of Enterotoxigenic Escherichia coli Reveals Structural Polymorphism.
J.Bacteriol., 195:1360-1370, 2013

PubMed Abstract: Enterotoxigenic Escherichia coli (ETEC) is a bacterial pathogen that causes diarrhea in children and travelers in developing countries. ETEC adheres to host epithelial cells in the small intestine via a variety of different pili. The CS1 pilus is a prototype for a family of related pili, including the CFA/I pili, present on ETEC and other Gram-negative bacterial pathogens. These pili are assembled by an outer membrane usher protein that catalyzes subunit polymerization via donor strand complementation, in which the N terminus of each incoming pilin subunit fits into a hydrophobic groove in the terminal subunit, completing a β-sheet in the Ig fold. Here we determined a crystal structure of the CS1 major pilin subunit, CooA, to a 1.6-Å resolution. CooA is a globular protein with an Ig fold and is similar in structure to the CFA/I major pilin CfaB. We determined three distinct negative-stain electron microscopic reconstructions of the CS1 pilus and generated pseudoatomic-resolution pilus structures using the CooA crystal structure. CS1 pili adopt multiple structural states with differences in subunit orientations and packing. We propose that the structural perturbations are accommodated by flexibility in the N-terminal donor strand of CooA and by plasticity in interactions between exposed flexible loops on adjacent subunits. Our results suggest that CS1 and other pili of this class are extensible filaments that can be stretched in response to mechanical stress encountered during colonization.

PubMed: 23175654
DOI: 10.1128/JB.01989-12

実験手法

X-RAY DIFFRACTION (1.6 Å)