4HID

Crystal Structure of Schizosaccharomyces pombe Pot1pC bound to ssDNA (GCTTACGGT)

4HID の概要

• エントリーDOI: 10.2210/pdb4hid/pdb
• 関連するPDBエントリー: 4HIK 4HIM 4HIO 4HJ5 4HJ7 4HJ8 4HJ9 4HJA
• 分子名称: Protection of telomeres protein 1, DNA (5'-D(*GP*CP*TP*TP*AP*CP*GP*GP*T)-3') (3 entities in total)
• 機能のキーワード: specificity, plasticity, promiscuity, ob-fold, dna-binding, telomeric dna, dna binding protein
• 由来する生物種: Schizosaccharomyces pombe (Fission yeast); 詳細
• 細胞内の位置: Nucleus: O13988
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 19929.22

構造登録者

Dickey, T.H.,Wuttke, D.S. (登録日: 2012-10-11, 公開日: 2012-12-12, 最終更新日: 2024-02-28)

主引用文献

Dickey, T.H.,McKercher, M.A.,Wuttke, D.S.
Nonspecific Recognition Is Achieved in Pot1pC through the Use of Multiple Binding Modes.
Structure, 21:121-132, 2013

PubMed Abstract: Pot1 is the protein responsible for binding to and protecting the 3' single-stranded DNA (ssDNA) overhang at most eukaryotic telomeres. Here, we present the crystal structure of one of the two oligonucleotide/oligosaccharide-binding folds (Pot1pC) that make up the ssDNA-binding domain in S. pombe Pot1. Comparison with the homologous human domain reveals unexpected structural divergence in the mode of ligand binding that explains the differing ligand requirements between species. Despite the presence of apparently base-specific hydrogen bonds, Pot1pC is able to bind a wide range of ssDNA sequences with thermodynamic equivalence. To address how Pot1pC binds ssDNA with little to no specificity, multiple structures of Pot1pC bound to noncognate ssDNA ligands were solved. These structures reveal that this promiscuity is implemented through new binding modes that thermodynamically compensate for base-substitutions through alternate stacking interactions and new H-bonding networks.

PubMed: 23201273
DOI: 10.1016/j.str.2012.10.015

実験手法

X-RAY DIFFRACTION (1.822 Å)