4HFO

Biogenic amine-binding protein selenomethionine derivative

4HFO の概要

• エントリーDOI: 10.2210/pdb4hfo/pdb
• 関連するPDBエントリー: 4GE1 4GET
• 分子名称: Biogenic amine-binding protein (1 entity in total)
• 機能のキーワード: beta barrel, serotonin, norepinephrine, salivary gland, amine-binding protein
• 由来する生物種: Rhodnius prolixus (Triatomid bug)
• タンパク質・核酸の鎖数: 8
• 化学式量合計: 177371.17

構造登録者

Andersen, J.F.,Xu, X.,Chang, B.,Mans, B.J.,Ribeiro, J.M. (登録日: 2012-10-05, 公開日: 2013-01-02, 最終更新日: 2024-10-16)

主引用文献

Xu, X.,Chang, B.W.,Mans, B.J.,Ribeiro, J.M.,Andersen, J.F.
Structure and ligand-binding properties of the biogenic amine-binding protein from the saliva of a blood-feeding insect vector of Trypanosoma cruzi.
Acta Crystallogr.,Sect.D, 69:105-113, 2013

PubMed Abstract: Proteins that bind small-molecule mediators of inflammation and hemostasis are essential for blood-feeding by arthropod vectors of infectious disease. In ticks and triatomine insects, the lipocalin protein family is greatly expanded and members have been shown to bind biogenic amines, eicosanoids and ADP. These compounds are potent mediators of platelet activation, inflammation and vascular tone. In this paper, the structure of the amine-binding protein (ABP) from Rhodnius prolixus, a vector of the trypanosome that causes Chagas disease, is described. ABP binds the biogenic amines serotonin and norepinephrine with high affinity. A complex with tryptamine shows the presence of a binding site for a single ligand molecule in the central cavity of the β-barrel structure. The cavity contains significant additional volume, suggesting that this protein may have evolved from the related nitrophorin proteins, which bind a much larger heme ligand in the central cavity.

PubMed: 23275168
DOI: 10.1107/S0907444912043326

実験手法

X-RAY DIFFRACTION (3 Å)