4HEX

A novel conformation of calmodulin

4HEX の概要

• エントリーDOI: 10.2210/pdb4hex/pdb
• 分子名称: Calmodulin, ZINC ION, CALCIUM ION, ... (4 entities in total)
• 機能のキーワード: calmodulin, novel conformation, trans, ef-hand motifs, calcium signalling, calcium binding, neuromodulin, neurogranin, calcium binding protein
• 由来する生物種: Mus musculus (mouse)
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 36178.02

構造登録者

Kumar, V.,Chichili, V.P.R.,Sivaraman, J. (登録日: 2012-10-04, 公開日: 2013-03-06, 最終更新日: 2024-03-20)

主引用文献

Kumar, V.,Chichili, V.P.R.,Tang, X.,Sivaraman, J.
A novel trans conformation of ligand-free calmodulin
Plos One, 8:e54834-e54834, 2013

PubMed Abstract: Calmodulin (CaM) is a highly conserved eukaryotic protein that binds specifically to more than 100 target proteins in response to calcium (Ca(2+)) signal. CaM adopts a considerable degree of structural plasticity to accomplish this physiological role; however, the nature and extent of this plasticity remain to be fully understood. Here, we report the crystal structure of a novel trans conformation of ligand-free CaM where the relative disposition of two lobes of CaM is different, a conformation to-date not reported. While no major structural changes were observed in the independent N- and C-lobes as compared with previously reported structures of Ca(2+)/CaM, the central helix was tilted by ~90° at Arg75. This is the first crystal structure of CaM to show a drastic conformational change in the central helix, and reveals one of several possible conformations of CaM to engage with its binding partner.

PubMed: 23382982
DOI: 10.1371/journal.pone.0054834

実験手法

X-RAY DIFFRACTION (2.001 Å)