4HE5

Crystal structure of the selenomethionine variant of the C-terminal domain of Geobacillus thermoleovorans putative U32 peptidase

4HE5 の概要

• エントリーDOI: 10.2210/pdb4he5/pdb
• 関連するPDBエントリー: 4HE6
• 分子名称: Peptidase family U32, SULFATE ION (3 entities in total)
• 機能のキーワード: ultra-tight crystal packing, unknown function
• 由来する生物種: Geobacillus thermoleovorans
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 10598.60

構造登録者

Trillo-Muyo, S.,Jasilionis, A.,Domagalski, M.J.,Chruszcz, M.,Minor, W.,Kuisiene, N.,Arolas, J.L.,Sola, M.,Gomis-Ruth, F.X. (登録日: 2012-10-03, 公開日: 2012-11-14, 最終更新日: 2022-04-13)

主引用文献

Trillo-Muyo, S.,Jasilionis, A.,Domagalski, M.J.,Chruszcz, M.,Minor, W.,Kuisiene, N.,Arolas, J.L.,Sola, M.,Gomis-Ruth, F.X.
Ultratight crystal packing of a 10 kDa protein.
Acta Crystallogr.,Sect.D, 69:464-470, 2013

PubMed Abstract: While small organic molecules generally crystallize forming tightly packed lattices with little solvent content, proteins form air-sensitive high-solvent-content crystals. Here, the crystallization and full structure analysis of a novel recombinant 10 kDa protein corresponding to the C-terminal domain of a putative U32 peptidase are reported. The orthorhombic crystal contained only 24.5% solvent and is therefore among the most tightly packed protein lattices ever reported.

PubMed: 23519421
DOI: 10.1107/S0907444912050135

実験手法

X-RAY DIFFRACTION (1.15 Å)