4HC9

DNA binding by GATA transcription factor-complex 3

4HC9 の概要

• エントリーDOI: 10.2210/pdb4hc9/pdb
• 関連するPDBエントリー: 4HC7 4HCA
• 分子名称: Trans-acting T-cell-specific transcription factor GATA-3, DNA (5'-D(*TP*TP*CP*CP*TP*AP*AP*AP*TP*CP*AP*GP*AP*GP*AP*TP*AP*AP*CP*C)-3'), DNA (5'-D(*AP*AP*GP*GP*TP*TP*AP*TP*CP*TP*CP*TP*GP*AP*TP*TP*TP*AP*GP*G)-3'), ... (5 entities in total)
• 機能のキーワード: zinc finger, gata transcription factor, dna bridging, transcription-dna complex, transcription/dna
• 由来する生物種: Homo sapiens (human); 詳細
• 細胞内の位置: Nucleus: P23771
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 25289.68

構造登録者

Chen, Y.,Bates, D.L.,Dey, R.,Chen, L. (登録日: 2012-09-28, 公開日: 2012-12-05, 最終更新日: 2024-02-28)

主引用文献

Chen, Y.,Bates, D.L.,Dey, R.,Chen, P.H.,Machado, A.C.,Laird-Offringa, I.A.,Rohs, R.,Chen, L.
DNA Binding by GATA Transcription Factor Suggests Mechanisms of DNA Looping and Long-Range Gene Regulation.
Cell Rep, 2:1197-1206, 2012

PubMed Abstract: GATA transcription factors regulate transcription during development and differentiation by recognizing distinct GATA sites with a tandem of two conserved zinc fingers, and by mediating long-range DNA looping. However, the molecular basis of these processes is not well understood. Here, we determined three crystal structures of the full DNA-binding domain (DBD) of human GATA3 protein, which contains both zinc fingers, in complex with different DNA sites. In one structure, both zinc fingers wrap around a palindromic GATA site, cooperatively enhancing the binding affinity and kinetic stability. Strikingly, in the other two structures, the two fingers of GATA DBD bind GATA sites on different DNA molecules, thereby bridging two separate DNA fragments. This was confirmed in solution by an in-gel fluorescence resonance energy transfer analysis. These findings not only provide insights into the structure and function of GATA proteins but also shed light on the molecular basis of long-range gene regulation.

PubMed: 23142663
DOI: 10.1016/j.celrep.2012.10.012

実験手法

X-RAY DIFFRACTION (1.6 Å)