4HBL

Crystal structure of AbfR of Staphylococcus epidermidis

4HBL の概要

• エントリーDOI: 10.2210/pdb4hbl/pdb
• 分子名称: Transcriptional regulator, MarR family (2 entities in total)
• 機能のキーワード: hth, transcription factor, dna binding, transcription
• 由来する生物種: Staphylococcus epidermidis
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 71265.86

構造登録者

Liu, X.,Sun, X.,Gan, J.,Lan, L.,Yang, C.-G. (登録日: 2012-09-28, 公開日: 2013-01-02, 最終更新日: 2013-02-27)

主引用文献

Liu, X.,Sun, X.,Wu, Y.,Xie, C.,Zhang, W.,Wang, D.,Chen, X.,Qu, D.,Gan, J.,Chen, H.,Jiang, H.,Lan, L.,Yang, C.G.
Oxidation-sensing Regulator AbfR Regulates Oxidative Stress Responses, Bacterial Aggregation, and Biofilm Formation in Staphylococcus epidermidis.
J.Biol.Chem., 288:3739-3752, 2013

PubMed Abstract: Staphylococcus epidermidis is a notorious human pathogen that is the major cause of infections related to implanted medical devices. Although redox regulation involving reactive oxygen species is now recognized as a critical component of bacterial signaling and regulation, the mechanism by which S. epidermidis senses and responds to oxidative stress remains largely unknown. Here, we report a new oxidation-sensing regulator, AbfR (aggregation and biofilm formation regulator) in S. epidermidis. An environment of oxidative stress mediated by H(2)O(2) or cumene hydroperoxide markedly up-regulates the expression of abfR gene. Similar to Pseudomonas aeruginosa OspR, AbfR is negatively autoregulated and dissociates from promoter DNA in the presence of oxidants. In vivo and in vitro analyses indicate that Cys-13 and Cys-116 are the key functional residues to form an intersubunit disulfide bond upon oxidation in AbfR. We further show that deletion of abfR leads to a significant induction in H(2)O(2) or cumene hydroperoxide resistance, enhanced bacterial aggregation, and reduced biofilm formation. These effects are mediated by derepression of SERP2195 and gpxA-2 that lie immediately downstream of the abfR gene in the same operon. Thus, oxidative stress likely acts as a signal to modulate S. epidermidis key virulence properties through AbfR.

PubMed: 23271738
DOI: 10.1074/jbc.M112.426205

実験手法

X-RAY DIFFRACTION (2.5 Å)