4H7A

Crystal structure of CasB from Thermus thermophilus

4H7A の概要

• エントリーDOI: 10.2210/pdb4h7a/pdb
• 分子名称: CRISPR-associated protein Cse2 (2 entities in total)
• 機能のキーワード: crispr, cascade, casb, crispr-assoicated protein, nucleic acid binding protein, dna binding protein
• 由来する生物種: Thermus thermophilus
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 38846.16

構造登録者

Ke, A.,Nam, K.H. (登録日: 2012-09-20, 公開日: 2012-10-03, 最終更新日: 2023-09-20)

主引用文献

Nam, K.H.,Huang, Q.,Ke, A.
Nucleic acid binding surface and dimer interface revealed by CRISPR-associated CasB protein structures.
Febs Lett., 586:3956-3961, 2012

PubMed Abstract: The CRISPR system is an adaptive RNA-based microbial immune system against invasive genetic elements. CasB is an essential protein component in Type I-E Cascade. Here, we characterize CasB proteins from three different organisms as non-specific nucleic acid binding proteins. The Thermobifida fusca CasB crystal structure reveals conserved positive surface charges, which we show are important for its nucleic acid binding function. EM docking reveals that CasB dimerization aligns individual nucleic acid binding surfaces into a curved, elongated binding surface inside Type I-E Cascade, consistent with the putative functions of CasB in ds-DNA recruitment and crRNA-DNA duplex formation steps.

PubMed: 23079036
DOI: 10.1016/j.febslet.2012.09.041

実験手法

X-RAY DIFFRACTION (2.6 Å)