4H3L

mPlum-E16P

4H3L の概要

• エントリーDOI: 10.2210/pdb4h3l/pdb
• 関連するPDBエントリー: 4H3M 4H3N
• 分子名称: Fluorescent protein plum, SODIUM ION (3 entities in total)
• 機能のキーワード: fluorescent protein
• 由来する生物種: Discosoma sp. LW-2004
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 53054.78

構造登録者

Moore, M.M.,Chica, R.A. (登録日: 2012-09-14, 公開日: 2012-10-31, 最終更新日: 2023-12-06)

主引用文献

Moore, M.M.,Oteng-Pabi, S.K.,Pandelieva, A.T.,Mayo, S.L.,Chica, R.A.
Recovery of Red Fluorescent Protein Chromophore Maturation Deficiency through Rational Design.
Plos One, 7:e52463-e52463, 2012

PubMed Abstract: Red fluorescent proteins (RFPs) derived from organisms in the class Anthozoa have found widespread application as imaging tools in biological research. For most imaging experiments, RFPs that mature quickly to the red chromophore and produce little or no green chromophore are most useful. In this study, we used rational design to convert a yellow fluorescent mPlum mutant to a red-emitting RFP without reverting any of the mutations causing the maturation deficiency and without altering the red chromophore's covalent structure. We also created an optimized mPlum mutant (mPlum-E16P) that matures almost exclusively to the red chromophore. Analysis of the structure/function relationships in these proteins revealed two structural characteristics that are important for efficient red chromophore maturation in DsRed-derived RFPs. The first is the presence of a lysine residue at position 70 that is able to interact directly with the chromophore. The second is an absence of non-bonding interactions limiting the conformational flexibility at the peptide backbone that is oxidized during red chromophore formation. Satisfying or improving these structural features in other maturation-deficient RFPs may result in RFPs with faster and more complete maturation to the red chromophore.

PubMed: 23285050
DOI: 10.1371/journal.pone.0052463

実験手法

X-RAY DIFFRACTION (1.65 Å)