4H13

Crystal Structure of the Cytochrome b6f Complex from Mastigocladus laminosus with TDS

4H13 の概要

• エントリーDOI: 10.2210/pdb4h13/pdb
• 関連するPDBエントリー: 1Q90 1VF5 2E76 4H0L 4H44
• 分子名称: Cytochrome b6, PROTOPORPHYRIN IX CONTAINING FE, (7R,17E)-4-HYDROXY-N,N,N,7-TETRAMETHYL-7-[(8E)-OCTADEC-8-ENOYLOXY]-10-OXO-3,5,9-TRIOXA-4-PHOSPHAHEPTACOS-17-EN-1-AMINIUM 4-OXIDE, ... (22 entities in total)
• 機能のキーワード: alpha helix, beta sheet, plastoquinol-plastocyanin oxidoreductase, plastocyanin, thylakoid membrane, photosynthesis
• 由来する生物種: Mastigocladus laminosus (Fischerella sp.); 詳細
• 細胞内の位置: Cellular thylakoid membrane; Multi-pass membrane protein: P83791 P83792; Cellular thylakoid membrane; Single-pass membrane protein: P83793 P83795 P83796 P83797 P83798; Cellular thylakoid membrane; Single-pass membrane protein (By similarity): P83794
• タンパク質・核酸の鎖数: 8
• 化学式量合計: 117209.61

構造登録者

Hasan, S.S.,Yamashita, E.,Baniulis, D.,Cramer, W.A. (登録日: 2012-09-10, 公開日: 2013-02-13, 最終更新日: 2024-11-20)

主引用文献

Hasan, S.S.,Yamashita, E.,Baniulis, D.,Cramer, W.A.
Quinone-dependent proton transfer pathways in the photosynthetic cytochrome b6f complex
Proc.Natl.Acad.Sci.USA, 110:4297-4302, 2013

PubMed Abstract: As much as two-thirds of the proton gradient used for transmembrane free energy storage in oxygenic photosynthesis is generated by the cytochrome b6f complex. The proton uptake pathway from the electrochemically negative (n) aqueous phase to the n-side quinone binding site of the complex, and a probable route for proton exit to the positive phase resulting from quinol oxidation, are defined in a 2.70-Å crystal structure and in structures with quinone analog inhibitors at 3.07 Å (tridecyl-stigmatellin) and 3.25-Å (2-nonyl-4-hydroxyquinoline N-oxide) resolution. The simplest n-side proton pathway extends from the aqueous phase via Asp20 and Arg207 (cytochrome b6 subunit) to quinone bound axially to heme c(n). On the positive side, the heme-proximal Glu78 (subunit IV), which accepts protons from plastosemiquinone, defines a route for H(+) transfer to the aqueous phase. These pathways provide a structure-based description of the quinone-mediated proton transfer responsible for generation of the transmembrane electrochemical potential gradient in oxygenic photosynthesis.

PubMed: 23440205
DOI: 10.1073/pnas.1222248110

実験手法

X-RAY DIFFRACTION (3.07 Å)