4GZY

Crystal structures of bacterial RNA Polymerase paused elongation complexes

4GZY の概要

• エントリーDOI: 10.2210/pdb4gzy/pdb
• 分子名称: DNA-directed RNA polymerase subunit alpha, DNA-directed RNA polymerase subunit beta, DNA-directed RNA polymerase subunit beta', ... (9 entities in total)
• 機能のキーワード: rna polymerase, transcription, paused transcription elongation complex, transcriptional pausing, dna directed rna transcription, transcription-dna-rna complex, transcription/dna/rna
• 由来する生物種: Thermus thermophilus; 詳細
• タンパク質・核酸の鎖数: 8
• 化学式量合計: 399529.51

構造登録者

Weixlbaumer, A.,Leon, K.,Landick, R.,Darst, S.A. (登録日: 2012-09-06, 公開日: 2013-02-13, 最終更新日: 2023-09-13)

主引用文献

Weixlbaumer, A.,Leon, K.,Landick, R.,Darst, S.A.
Structural basis of transcriptional pausing in bacteria.
Cell(Cambridge,Mass.), 152:431-441, 2013

PubMed Abstract: Transcriptional pausing by multisubunit RNA polymerases (RNAPs) is a key mechanism for regulating gene expression in both prokaryotes and eukaryotes and is a prerequisite for transcription termination. Pausing and termination states are thought to arise through a common, elemental pause state that is inhibitory for nucleotide addition. We report three crystal structures of Thermus RNAP elemental paused elongation complexes (ePECs). The structures reveal the same relaxed, open-clamp RNAP conformation in the ePEC that may arise by failure to re-establish DNA contacts during translocation. A kinked bridge-helix sterically blocks the RNAP active site, explaining how this conformation inhibits RNAP catalytic activity. Our results provide a framework for understanding how RNA hairpin formation stabilizes the paused state and how the ePEC intermediate facilitates termination.

PubMed: 23374340
DOI: 10.1016/j.cell.2012.12.020

実験手法

X-RAY DIFFRACTION (3.5054 Å)