4GYN
The E142L mutant of the amidase from Geobacillus pallidus
Summary for 4GYN
Entry DOI | 10.2210/pdb4gyn/pdb |
Related | 4GYL |
Descriptor | Aliphatic amidase, CHLORIDE ION (3 entities in total) |
Functional Keywords | amidase, catalytic tetrad, amidase mechanism, hydrolase |
Biological source | Bacillus sp. |
Total number of polymer chains | 1 |
Total formula weight | 38660.33 |
Authors | Weber, B.W.,Sewell, B.T.,Kimani, S.W.,Varsani, A.,Cowan, D.A.,Hunter, R. (deposition date: 2012-09-05, release date: 2013-08-21, Last modification date: 2023-11-08) |
Primary citation | Weber, B.W.,Kimani, S.W.,Varsani, A.,Cowan, D.A.,Hunter, R.,Venter, G.A.,Gumbart, J.C.,Sewell, B.T. The mechanism of the amidases: mutating the glutamate adjacent to the catalytic triad inactivates the enzyme due to substrate mispositioning. J.Biol.Chem., 288:28514-28523, 2013 Cited by PubMed: 23946488DOI: 10.1074/jbc.M113.503284 PDB entries with the same primary citation |
Experimental method | X-RAY DIFFRACTION (1.9 Å) |
Structure validation
Download full validation report![Download](/newweb/media/icons/dl.png)
![Download](/newweb/media/icons/dl.png)