4GYL

The E142L mutant of the amidase from Geobacillus pallidus showing the result of Michael addition of acrylamide at the active site cysteine

4GYL の概要

• エントリーDOI: 10.2210/pdb4gyl/pdb
• 関連するPDBエントリー: 4GYN
• 分子名称: Aliphatic amidase, PROPIONAMIDE, CHLORIDE ION, ... (4 entities in total)
• 機能のキーワード: amidase, catalytic tetrad, amidase mechanism, acrylamide, michael adduct, hydrolase
• 由来する生物種: Bacillus sp.
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 38733.42

構造登録者

Weber, B.W.,Sewell, B.T.,Kimani, S.W.,Varsani, A.,Cowan, D.A.,Hunter, R. (登録日: 2012-09-05, 公開日: 2013-08-21, 最終更新日: 2014-02-05)

• 主引用文献: Weber, B.W.,Kimani, S.W.,Varsani, A.,Cowan, D.A.,Hunter, R.,Venter, G.A.,Gumbart, J.C.,Sewell, B.T.; The mechanism of the amidases: mutating the glutamate adjacent to the catalytic triad inactivates the enzyme due to substrate mispositioning.; J.Biol.Chem., 288:28514-28523, 2013; PubMed: 23946488; DOI: 10.1074/jbc.M113.503284

実験手法

X-RAY DIFFRACTION (1.9 Å)