4GYG

Crystal structure of the Rio2 kinase from Chaetomium thermophilum

4GYG の概要

• エントリーDOI: 10.2210/pdb4gyg/pdb
• 関連するPDBエントリー: 4GYI
• 分子名称: Rio2 kinase (2 entities in total)
• 機能のキーワード: rio-type ser/thr protein kinase, ribosome biogenesis factor, pre-40s maturation, ribosomal rna processing, pre-40s, ribosomal rna, phosphorylation, transferase
• 由来する生物種: Chaetomium thermophilum
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 44691.84

構造登録者

Ferreira-Cerca, S.,Sagar, V.,Schafer, T.,Diop, M.,Wesseling, A.M.,Lu, H.,Chai, E.,Hurt, E.,LaRonde-LeBlanc, N. (登録日: 2012-09-05, 公開日: 2012-10-17, 最終更新日: 2023-09-13)

主引用文献

Ferreira-Cerca, S.,Sagar, V.,Schafer, T.,Diop, M.,Wesseling, A.M.,Lu, H.,Chai, E.,Hurt, E.,Laronde-Leblanc, N.
ATPase-dependent role of the atypical kinase Rio2 on the evolving pre-40S ribosomal subunit.
Nat.Struct.Mol.Biol., 19:1316-1323, 2012

PubMed Abstract: Ribosome synthesis involves dynamic association of ribosome-biogenesis factors with evolving preribosomal particles. Rio2 is an atypical protein kinase required for pre-40S subunit maturation. We report the crystal structure of eukaryotic Rio2-ATP-Mg(2+) complex. The active site contains ADP-Mg(2+) and a phosphoaspartate intermediate typically found in Na(+), K(+) and Ca(2+) ATPases but not protein kinases. Consistent with this finding, ctRio2 exhibits a robust ATPase activity in vitro. In vivo, Rio2 docks on the ribosome, with its active site occluded and its flexible loop positioned to interact with the pre-40S subunit. Moreover, Rio2 catalytic activity is required for its dissociation from the ribosome, a necessary step in pre-40S maturation. We propose that phosphoryl transfer from ATP to Asp257 in Rio2's active site and subsequent hydrolysis of the aspartylphosphate could be a trigger to power late cytoplasmic 40S subunit biogenesis.

PubMed: 23104056
DOI: 10.1038/nsmb.2403

実験手法

X-RAY DIFFRACTION (2.482 Å)